Analysis of bud morphology.
收藏Figshare2015-12-02 更新2026-04-29 收录
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Log-phase cultures of the indicated cell types were incubated at 37°C for 24 hours or 4 hours in liquid medium, 150 RPM. DIC images of the cultures were analyzed for mother and daughter cell morphology. For 4 hour cultures, a sufficient number of cells (nt) was examined to identify comparable numbers of budding cells (nb). Abnormal buds included those that were non-round or had wide bud necks, and mother cells with multiple buds or buds that were abnormally large (>75% of the size of the mother cell).
创建时间：
2015-12-02
