Analysis of bud morphology.

Log-phase cultures of the indicated cell types were incubated at 37°C for 24 hours or 4 hours in liquid medium, 150 RPM. DIC images of the cultures were analyzed for mother and daughter cell morphology. For 4 hour cultures, a sufficient number of cells (nt) was examined to identify comparable numbers of budding cells (nb). Abnormal buds included those that were non-round or had wide bud necks, and mother cells with multiple buds or buds that were abnormally large (>75% of the size of the mother cell).