DNA and mRNA levels of CPV1 measured by Real-Time PCR.
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mRNA was extracted only from those samples whose DNA was tested (5 out of 15 for VPs; 5 out of 15 for SPs and 8 out of 33 for SCCs). E4, E7, L1 and L2 probes were used to detect CPV1 DNA and E4, E7 and L1 probes were suitable for viral mRNA detection. Ct values were normalized with housekeeping 18S. In the table Ct values are shown as the means ± standard deviation. Not detectable Ct are indicated as ND. The positive (PC) and negative controls (NC) ensured the specificity of the results. No Template Controls were included on every RNA plate for every probe to check for contamination.DNA and mRNA levels of CPV1 measured by Real-Time PCR.
创建时间：
2015-12-02
