Next Generation sequencing revealed the quantitative transcriptome analysis of the preimplantation embryos (3 d.p.c) generated from the cross between L13a+/- mice

Comparative study of the genomewide transcriptome analysis with reference to the regression scale of ribosomal protein L13a transcript level i.e in the absence of L13a (-/-), intermediate level of L13a (+/-) and wild type (+/+) Preimplanted embryos at the morula stage were collected by flashing the oviduct/uterus of pregnant L13a(+/-) mice at 3 d.p.c Each embryos were washed in PBS and transferred to 10ml of Cells-to-CDNA II Cell lysis buffer (Cat#AM8723 invitrogen) and boiled at 75 degree Centrigrade for 10 minutes, lysates were centrifuged briefly and transferred immediately on ice. RNA purification was performed using PicoPure RNA isolation it (Cat# KIT0214) following users manual. These samples were submitted for library construction and next generation sequencing.