Expression data from Control or ShSuz12 rat Intestinal epithelial cells IEC-6

Polycomb-group proteins form multimeric protein complexes involved in transcriptional silencing. The Polycomb Repressive complex 2 (PRC2) contains the Suppressor of Zeste-12 protein (Suz12) and the histone methyltransferase Enhancer of Zeste protein-2 (Ezh2). This complex, catalyzing the di- and tri-methylation of histone H3 lysine 27, is essential for embryonic development and stem cell renewal. However, the role of Polycomb-group protein complexes in the control of the intestinal epithelial cell (IEC) phenotype is not known. We investigated the impact of Suz 12 depletion on gene expression in IEC-6 cells.多梳家族蛋白（Polycomb-group proteins）可形成参与转录沉默的多聚体蛋白质复合物。多梳抑制复合物2（Polycomb Repressive Complex 2，PRC2）包含Zeste抑制因子12蛋白（Suppressor of Zeste-12 protein，Suz12）以及组蛋白甲基转移酶Zeste增强子蛋白2（Enhancer of Zeste protein-2，Ezh2）。该复合物可催化组蛋白H3赖氨酸27的二甲基化与三甲基化，对胚胎发育及干细胞自我更新至关重要。然而，多梳家族蛋白复合物在调控肠上皮细胞（intestinal epithelial cell，IEC）表型中的作用尚未明确。本研究探究了Suz12敲降对IEC-6细胞基因表达的影响。