Identification of gene deletions affecting transcription directionality at the GCG1/SUT098 bidirectional promoter

The synthetic genetic array (SGA) technology was used to find non-essential genes which affect divergent transcription from bidirectional promoters in yeast S.cerevisiae.
Conclusion:
Genetic modifiers of the GCG1/SUT098 promoter directionality were identified.
Notes:
Library of non-essential gene deletions was crossed to fluorescent protein reporter (FPR) strain expressing YFP and mCherry proteins from the bidirectional promoter. Gene deletions affecting the directionality of divergent transcription are expected to change the ratio of YFP to mCherry fluorescence. Negative control samples (wild type BY4741) and positive control samples (BY4741 carrying the fluorescence reporter construct) were included into each 384-well plate.