Data_Sheet_1_Lytic Bacteriophage EFA1 Modulates HCT116 Colon Cancer Cell Growth and Upregulates ROS Production in an Enterococcus faecalis Co-culture System.PDF

Enterococcus faecalis is an opportunistic pathogen in the gut microbiota that’s associated with a range of difficult to treat nosocomial infections. It is also known to be associated with some colorectal cancers. Its resistance to a range of antibiotics and capacity to form biofilms increase its virulence. Unlike antibiotics, bacteriophages are capable of disrupting biofilms which are key in the pathogenesis of diseases such as UTIs and some cancers. In this study, bacteriophage EFA1, lytic against E. faecalis, was isolated and its genome fully sequenced and analyzed in silico. Electron microscopy images revealed EFA1 to be a Siphovirus. The bacteriophage was functionally assessed and shown to disrupt E. faecalis biofilms as well as modulate the growth stimulatory effects of E. faecalis in a HCT116 colon cancer cell co-culture system, possibly via the effects of ROS. The potential exists for further testing of bacteriophage EFA1 in these systems as well as in vivo models.

粪肠球菌（Enterococcus faecalis）作为一种肠道微生物群中的机会性病原体，与一系列难以治疗的医院感染相关，亦已知与某些结直肠癌有关。其对多种抗生素的耐药性及其形成生物膜的能力，加剧了其致病力。与抗生素不同，噬菌体能够破坏生物膜，后者在尿路感染（UTIs）和一些癌症的发病机制中起着关键作用。在本研究中，从粪肠球菌中分离出的噬菌体EFA1，对粪肠球菌具有裂解作用，其基因组已完全测序并在计算机上进行分析。电子显微镜图像显示EFA1为细长噬菌体病毒（Siphovirus）。噬菌体的功能评估表明，它能够破坏粪肠球菌的生物膜，并在HCT116结直肠癌细胞共培养系统中调节粪肠球菌的生长促进作用，可能通过活性氧（ROS）的作用实现。噬菌体EFA1在这些系统和体内模型中进一步测试的潜力存在。