Validation of several RNA targets of RNase III.

The annotation of the genome is taken from strain N315.(a)Strand (+/−) of the genome;(b)Experiments were performed in RN6390 strain and the mutant Δrnc strain transformed with a plasmid expressing the wt enzyme (wt, strain EL79), the mutant D63A (strain EL80), or E135A enzyme (strain EL78); nd is for not detected.(c)References: (1) Abu-Qatouseh et al. [36]; (2) Beaume et al. [37]; (3) Pichon and Felden [42]; (4) Geissmann et al. [43]; (5) Novick et al. [44]; (6) Huntzinger et al. [23]. N: No cleavage or binding detected; Y: cleavage (or binding) detected.*Assays were done in the presence or absence of the respective ligand. RNase III cleavage and binding assays were done as described in Materials and Methods.