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Zc3h10 controls pre-mRNA profile in preadipocytes

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https://www.ncbi.nlm.nih.gov/sra/SRP254946
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NGS was performed to understand the impact of Zc3h10 on pre-mRNA expression profile in C3H10T1/2 preadipocytes. Overall design: Cells were transduced after 24 hours they reached confluency with adenoviral vectors carrying Scramble and ShZc3h10 sequences. After 36h cells were labelled with 4-thiouridine 300µM for 10 minutes and then total RNA was isolated. Labelled pre-mRNA was subsequently isolated by biotin-streptavidin method.
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2021-04-09
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