Urinary bladder in constant darkness: time course

Analysis of urinary bladder in wild-type C57BL/6 females sacrificed every 4 hours at six time points under constant darkness after acclimation for 2 weeks under 12-hour light and 12-hour dark conditions. Results provide insight into circadian gene expression patterns in normal urinary bladder. Analysis of urinary bladder in wild-type C57BL/6 females sacrificed every 4 hours at six time points (n=2 for each time (CT 0, 4, 8, 12 and 20)) under constant darkness after acclimation for 2 weeks under 12-hour light and 12-hour dark conditions.本研究针对野生型C57BL/6雌性小鼠的膀胱（urinary bladder）组织展开分析：实验对象先在12小时光照与12小时黑暗交替的光周期条件下适应驯化2周，随后转移至持续黑暗环境中，按每4小时1个时间点的频率设置6个采样时点，对小鼠进行剖杀。该研究结果可为解析正常膀胱组织的昼夜节律基因表达模式提供理论参考。 另有针对野生型C57BL/6雌性小鼠膀胱组织的平行分析：实验对象经相同的12小时光照/12小时黑暗光周期条件适应驯化2周后转入持续黑暗环境，同样按每4小时1个时间点设置6个采样时点，每个时点样本量为2，对应昼夜时间（Circadian Time，CT）0、4、8、12及20，完成剖杀。