Massively parallel engineering of persistent CAR-Ts [GEO_CLASH_MIPS]

To identify immune-related gene perturbations that enhance CAR-T cell persistence in in vitro and in vivo settings, we generated thousands of CAR-T variants using the CLASH platform and evaluated crRNA representation across time in in vitro and in vivo cancer models. Overall design: MIPS probe were designed according to previously published protocols,(https://github.com/shendurelab/MIPGEN)