Identification of state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles

We performed quantitative label-free quantitative mass spectrometry (LFQ-MS) on 16 cell lysates and 16 EV lysates derived from BV2 microglia. These included four groups of BV2 cells (n=4/group) that were treated (72 hours) with either LPS (100ng/mL) to polarize to a pro-inflammatory state, IL-10 (50ng/mL) to polarize to a protective state, TGFβ (50ng/mL) to polarize to a homeostatic state or untreated controls