Supplementary material: Transcriptome analysis of fucoidan and L-fucose degradation by Pontiella desulfatans F1(T)

We examined the gene expression of the marine anaerobe Pontiella desulfatans F1(T) during growth on fucoidan from Fucus vesiculosus and on its monomer L-fucose with transcriptomics to obtain insight into the pathways involved. We observed upregulation of fucoidan-specific polysaccharide utilization loci (PULs), and found evidence for a novel L-fucose degradation pathway.

本研究通过转录组学（transcriptomics）技术，对生长于泡叶藻（Fucus vesiculosus）来源岩藻聚糖（fucoidan）及其单体L-岩藻糖（L-fucose）的海洋厌氧菌脱硫酸Pontiella菌株F1(T)的基因表达情况进行了检测，以解析其所涉及的代谢通路。研究观察到岩藻聚糖特异性多糖利用基因座（PULs，polysaccharide utilization loci）的上调表达，并发现了一条新型L-岩藻糖降解通路的相关证据。