Identification Of Significant Klh-Derived Peptide - Fig. S2

Supplemental figure 2 (S2) of the manuscript “Surface LAMP-2 is an endocytic receptor that diverts antigen internalized by human dendritic cells into highly immunogenic exosomes” by Dario A. Leone et al. (Journal of Immunology). ProPresent® antigen presentation assay from ProImmune was used here to identify potential differences in the presentation of immunogenic regions in Keyhole Limpet Hemocyanin (KLH), compared to KLH conjugated to anti-LAMP2 antibody (H4B4*KLH). The peptide eluted from HLA-DR of MoDC isolated from four different donors were identified using mass spectrometry (LCMSMS)-based analysis in order to identify the putative immunogenic peptides from KLH and two endogenous protein – myeloperoxidase(MPO) and heat shock protein 70 (HSc70).