A video tracking based improvement of acute toxicity test on Artemia salina

Booster biocides have negative impacts on marine organisms and it is therefore essential to monitor their toxicity prior to their use in antifouling paints. In this study, we describe a method for improving the reliability of a well-used acute toxicity test. We studied a model organism, Artemia salina, by using a novel video tracking method. Acute toxicities of a reference toxicant (potassium dichromate) and a potential toxicant (p-coumaric acid) were examined for newly hatched A. salina nauplii. Survival percentage, swimming velocity, and paths covered by nauplii were determined with the new algorithm. A. salina nauplii were affected by both tested toxicants, an outcome clearly detected by the algorithm. The results demonstrate that the video tracking algorithm could be used for testing the acute toxicities of booster biocides as well as other potential toxicants on A. salina. With slight modifications, it could be used for testing other similar aquatic micro-organisms. This report has linked video sequences.增效杀生剂对海洋生物具有负面影响，因此在将其用于防污涂料之前，有必要对其毒性进行监测。本研究提出一种可提升常用急性毒性测试可靠性的方法。本研究以卤虫（Artemia salina）作为模式生物，采用新型视频追踪技术开展实验。针对新孵化的卤虫无节幼体，本研究测试了参比毒物重铬酸钾（potassium dichromate）与潜在毒物对香豆酸（p-coumaric acid）的急性毒性。通过新开发的算法，可测定无节幼体的存活率、游泳速度与运动路径。两种受试毒物均会对卤虫无节幼体产生影响，该结果可通过本算法清晰检测得到。实验结果表明，该视频追踪算法可用于测试增效杀生剂及其他潜在毒物对卤虫的急性毒性。经小幅调整后，该算法亦可用于测试其他同类水生微生物的毒性。本报告附带相关视频序列。