Cardiac adventitial leptin receptor-expressing fibroblasts have a unique epigenetic landscape [mouse ATAC-Seq]

We performed ATAC (Assay for Transposase-Accessible Chromatin) sequencing of interstitial (LepR-Cre-) and adventitial (LepR-Cre+) cardiac fibroblasts in both the neonatal period (P7) and adulthood (P56). These ATAC-seq studies revealed that at P7 adventitial LepR-Cre+ cardiac fibroblasts and interstital LepR-Cre- cardiac fibroblasts exhibited abundant epigenomic differences. Overall design: ATAC-seq analyses were performed on FACS purified LepR-Cre+ and LepR-Cre- cardiac fibroblasts at P7 and P56.