RNA-seq transcriptonal profiling in partial hepatectomy liver tissues in Arid1a WT and liver specific KO mice

Arid1a is the subunit of SWI/SNF complex, which was reported to guide SWI/SNF to DNA. Here, we found that loss of Arid1a in the liver results in improved liver regeneration after partial hepatectomy.Genome-wide analysis showed that after hepatectomy,loss of Arid1a reduces the recruitment and activity of E2F4 on target promoters, resulting in expression programs that favor regeneration during injury.RNAseq shows transcriptional profiling in WT and Arid1a LKO livers pre- and post-hepatectomy, which confirmed the E2F4 target genes and cell cycle genes were upregulated after hepatectomy. After partial hepatectomy, liver transcriptional profiling in WT and Arid1a liver specific KO mice were generated by RNA-seq analysis.Arid1a是SWI/SNF复合物（SWI/SNF complex）的亚基，既往研究表明其可引导SWI/SNF复合物靶向结合DNA。本研究发现，肝脏中Arid1a缺失可改善部分肝切除术后的肝脏再生能力。全基因组分析显示，肝切除术后，Arid1a缺失会降低E2F4在靶基因启动子区域的招募效率与转录活性，进而激活利于损伤后再生的基因表达程序。转录组测序（RNA-seq）对肝切除术前及术后的野生型（Wild Type，WT）和Arid1a肝脏特异性敲除（Liver-specific Knockout，LKO）小鼠肝脏进行了转录谱分析，证实肝切除术后E2F4靶基因与细胞周期基因的表达水平显著上调。本研究通过RNA-seq分析，获取了部分肝切除术后野生型与Arid1a肝脏特异性敲除小鼠的肝脏转录组数据。