Supplement and refinement the standard and analysis of primary human hepatocytes.

We use high-throughout single-cell RNA sequencing technology to analyze primary human hepatocytes, combining with LC-MS/MS for drug-metabolizing function research. We measure the enzymatic metabolic function of PHH and perform a correlation analysis with the values documented in the quality inspection report. Furthermore, we assess functions beyond cellular metabolism as specified in the PHH standards. These additional functions include bile secretion, albumin (ALB) secretion, and cell purity, which are not addressed in the quality inspection report. Additionally, we examine the impact of donor characteristics such as body weight, age, underlying health conditions, and lifestyle habits (including smoking and alcohol consumption) on cellular function. We aim to conduct a comprehensive analysis of the factors that influence cellular function. This research not only seeks to supplement and refine the standards of PHH but also aspires to provide a crucial reference for the application of PHH in the domains of disease research and drug development in the future. scRNA-seq of six donors of primary human hepatocytes to profile gene expression and facilitate the verification of cell purity and asses differences in drug-metabolizing enzyme function.