Establishment and evaluation of a multiplexPCR amplification-based sequencing methodfor respiratory virus type A: Supplementary data

Aim: This study aimed to establish a simple whole-genome sequencing method for respiratory virus typeA (RSVA) used in genomic epidemiological studies. Methods: Multiple primers were designed to amplifyoverlapping amplicons specific to RSVA. The amplicons were sequenced using Illumina and Nanoporesequencing platforms and the sequencing performances of the two platforms were also compared.Results: The study shows that the developed method could recover almost full genomes of RSVA whenCt values were less than 33 for clinical samples. Conclusion: In this study, a whole-genome sequencingmethod was developed for RSVA based on multiplex PCR.