Data from: Conservation genetics of redside dace (Clinostomus elongatus): phylogeography and contemporary spatial structure

Redside dace Clinostomus elongatus (Teleostei: Cyprinidae) is a species of conservation concern that is declining throughout its range as a result of habitat fragmentation, degradation and loss. We characterized the genetic structure and diversity of redside dace populations across the species range using mitochondrial and microsatellite data to inform conservation efforts and assess how historical and recent events have shaped genetic structure and diversity within and among populations. Phylogeographic structure among 28 redside dace populations throughout southern Ontario (Canada) and the United States was assessed by sequence analysis of the mitochondrial cytochrome b and ATPase 6 and 8 genes. Populations were also genotyped using 10 microsatellite loci to examine genetic diversity within and among populations as well as contemporary spatial structuring. Mitochondrial DNA sequence data revealed three geographically distinct lineages, which were highly concordant with groupings identified by microsatellite analysis. The combined genetic data refute published glacial refugia hypotheses of a single Mississippian refugium or of two lineages associated with Mississippian and Atlantic refugia. Secondary contact between the two eastern groups was documented in the Allegheny River drainage and tributaries to Lake Ontario. With the exception of several allopatric populations within the Allegheny watershed, high genetic structuring among populations suggests their isolation, indicating that recovery efforts should be population-based.红侧带雅罗鱼（Clinostomus elongatus），隶属于硬骨鱼纲（Teleostei）、鲤科（Cyprinidae），是一种受保护关注物种，其整个分布范围内的种群因栖息地破碎化、退化与丧失而持续衰减。本研究利用线粒体DNA与微卫星（microsatellite）数据，对该物种全域分布范围内的红侧带雅罗鱼种群的遗传结构与遗传多样性进行了表征，以期为保护实践提供科学依据，并解析历史与近现代事件如何塑造种群内部及种群间的遗传结构与多样性。研究通过对线粒体细胞色素b（cytochrome b）以及ATP合酶6、8基因进行序列分析，对加拿大安大略省南部与美国境内共计28个红侧带雅罗鱼种群的系统地理结构开展了评估；同时，本研究采用10个微卫星位点对各种群进行基因分型，以解析种群内部、种群间的遗传多样性，以及当代空间遗传结构。线粒体DNA序列数据揭示了3个地理分化显著的支系，其聚类结果与微卫星分析得到的种群分组高度契合。整合后的遗传数据驳斥了已发表的两类冰期避难所（glacial refugia）假说：即仅存在单一密西西比避难所，或是存在与密西西比、大西洋避难所相关的两个支系的假说。在阿勒格尼河（Allegheny River）流域与安大略湖支流区域，研究人员记录到了东部两个支系的次生接触现象。除阿勒格尼流域内的数个异域种群外，种群间显著的遗传结构分化表明其彼此隔离，这提示该物种的恢复工作应以种群为核心单元开展。