Gsx2 Regulates Oligodendrocyte Precursor Formation in the Zebrafish Spinal Cord

In the spinal cord, neural progenitor cells (NPCs) of the pMN domain produce neurons in early development followed by oligodendrocytee precursor cells (OPCs), which subsequently differentiate as oligodendrocytes (OLs), the myelinating glia of the central nervous system. The mechanisms that specify neural progenitor cells to the OL lineage are not yet well understood. Using zebrafish, we combined single-cell Multi-omics and in vivo genetic approaches to identify gsx2 as a regulator of OPC specification. Overall design: Multi-ome seq: Cells were extracted from the trunks of wildtype zebrafish larvae expressing the olig2:egfp transgene at 36 and 48 hours post fertilization. Cells were FAC sorted for EGFP and nuclei were isolated. Joint scRNA-seq and scATAC-seq libraries were produced using the 10X Genomics platform.