Optical tweezers data for "Optimizing DNA origami assembly by reducing off-target interactions", by Shirt-Ediss et. al.

Optical tweezers data provided as MATLAB structures, one for each DNA origami type (T1–T3). Each field (N1, N2, ...) corresponds to an individual experiment. For each experiment, three equal-length vectors are provided: force - the measured force, in units of pNextension - the end-to-end molecular extension, in units of nmopenednuc - the estimated number of unpaired nucleotides (unitless) The vectors are aligned element-wise, such that each index corresponds to a single measurement point along the force–extension trajectory. Data are recorded sequentially at a sampling rate of 2.5 kHz. The number of open nucleotides is not directly measured, but inferred from the extension as follows: the contribution of the dsDNA handles is subtracted using an extensible worm-like chain (WLC) model. The remaining extension is attributed to ssDNA and converted to the number of nucleotides by dividing by the force-dependent ssDNA extension per nucleotide, also described by a WLC model. Full details of this procedure and model parameters are provided in the associated publication.