Radiosensitizing effect of THBS1 knockdown in radioresistant head and neck carcinoma cell lines

This study aimed to investigate the radiosensitizing effects of THBS1 knockdown in radioresistant head and neck squamous cell carcinoma (HNSCC) cell lines. Using siRNA-mediated silencing of THBS1 in CAL27 and Detroit 562 cells, we examined transcriptomic changes associated with enhanced sensitivity to proton beam irradiation. Bulk RNA-seq was performed to profile genome-wide expression patterns in control and THBS1-depleted cells, providing insights into gene expression programs linked to THBS1-mediated radioresistance and potential therapeutic targets. Overall design: RNA-seq profiling of two radioresistant HNSCC cell lines, CAL27 and Detroit 562, and their siRNA-mediated THBS1 knockdown derivatives, with three biological replicates per group, totaling 12 samples.