Phenotypes of EHEC strains isolated from patients with HUS in the Czech Republic.

aEHEC-Hly, EHEC hemolysin production; α-Hly, α hemolysin production; growth on CT-SMAC, indicator of tellurite resistance; urease, urease production; SMAC, utilization of sorbitol on sorbitol MacConkey agar; SOR, utilization of sorbitol (API 20E); RHA, utilization of rhamnose (API 20E); LDC, production of lysine decarboxylase; GLR, production of ß-D-glucuronidase. bThe highest dilution of culture supernatant which caused cytotoxicity in 50% Vero cells after 3 days. cProduction of Stx1 and Stx2 tested using the VTEC - RPLA kit. d−, the phenotype was absent; +, the phenotype was present (the numbers in parentheses indicated numbers of positive strains in the case that not all strains expressed the respective phenotype). eone O26:H11 and O172:NM and Orough:NM strains did not express EHEC-hlyA gene.