Impact of Vitamine D depletion and repletion on rat skeletal muscle

15 months Old Wistar Rat were submitted to 3 differents diets: control, without vitamin D and without vitamin D followed by a supplementation in vitamin D. At sacrifice, skeletal muscle were recovered, quickly frozen in liquid nitrogen. RNA profiling by microarrays was performed.选取15月龄的Wistar大鼠（Wistar Rat），将其分为三组，分别接受三种不同膳食干预：对照组、维生素D缺乏组，以及先开展维生素D缺乏膳食处理再辅以维生素D补充的组别。实验处死时采集骨骼肌样本，迅速置于液氮中速冻保存。后续采用微阵列（microarrays）技术完成RNA表达谱分析。