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Time course of the Arabidopsis root stem cells

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干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=d3098f7f041cfb09ac5ad9afeb1d502c
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The goal of this dataset was to transcriptionally profile the Arabidopsis root stem cells over developmental time.To accomplish this, we protoplasted roots expressing PET111:GFP every 8 hours from 4 days to 6 days old. We then collected GFP negative cells from the PET111:GFP line using cell sorting, as these cells represent the stem cell population. RNA was extracted from cells, libraries were prepared, and RNA-seq was performed. FPKM values were calulcated using Cufflinks.
提供机构:
Iowa State University
创建时间:
2022-02-20
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