Identification of novel transcription factors that regulate prostate cancer cell metabolism

To understand of the impact of KLF5 and NFYA on metabolism in prostate cancer cells LNCaP cells were plated in RPMI 1640 media with no phenol red and with 5% charcoal stripped serum, sodium pyruvate, penicillin and streptomycin. After 48h (to allow adnrogen deprivation), fresh media was added, with 96% ethanol or the synthetic androgen R1881 (10nM concentration). After trypsinization cells were seeded in media with CSS and transfected with a pool of non-targeted control siRNAs or a pool of siRNAs targeted against KLF5 and NFYA