Biomass of mesozooplankton in the western part of the Black Sea in 1997 during the Cooperative Marine Science Program for the Black Sea (CoMSBlack)

The "15BO1997001" dataset is based on samples collected in the spring of 1997. The whole dataset is composed of 66 samples (from 27 stations of National Monitoring Sampling Grid) with data of zooplankton species composition, abundance and biomass. Samples were collected in discrete layers 0-10, 0-20, 0-50, 10-25, 25-50, 50-100 and from bottom up to the surface at depths depending on water column stratification and the thermocline depth. The collected material was analysed using the method of Dimov (1959). Samples were brought to volume of 25-30 ml depending upon zooplankton density and mixed intensively until all organisms were distributed randomly in the sample volume. After that 5 ml of sample was taken and poured in the counting chamber which is a rectangle form for taxomomic identification and count. Large (> 1 mm body length) and not abundant species were calculated in whole sample. Counting and measuring of organisms were made in the Dimov chamber under the stereomicroscope to the lowest taxon possible. Taxonomic identification was done at the Institute of Oceanology by Asen Konsulov using the relevant taxonomic literature (Mordukhay-Boltovskoy, F.D. (Ed.). 1968, 1969,1972 ). The biomass was estimated as wet weight by Petipa, 1959 (based on species specific wet weight). Wet weight values were transformed to dry weight using the equation DW=0.16*WW as suggested by Vinogradov & Shushkina, 1987. The collected material was analysed using the method of Dimov (1959). Samples were brought to volume of 25-30 ml depending upon zooplankton density and mixed intensively until all organisms were distributed randomly in the sample volume. After that 5 ml of sample was taken and poured in the counting chamber which is a rectangle form for taxomomic identification and count. Copepods and Cladoceras were identified and enumerated; the other mesozooplankters were identified and enumerated at higher taxonomic level (commonly named as mesozooplankton groups). Large (> 1 mm body length) and not abundant species were calculated in whole sample. Counting and measuring of organisms were made in the Dimov chamber under the stereomicroscope to the lowest taxon possible. Taxonomic identification was done at the Institute of Oceanology by Asen Konsulov using the relevant taxonomic literature (Mordukhay-Boltovskoy, F.D. (Ed.). 1968, 1969,1972 ). The biomass was estimated as wet weight by Petipa, 1959 ussing standard average weight of each species in mg/m3. WW were converted to DW by equation DW=0.16*WW (Vinogradov ME, Sushkina EA, 1987)."15BO1997001"数据集基于1997年春季采集的样本构建。整套数据集共包含66份样本，取自国家监测采样网格的27个站位，涵盖浮游动物（zooplankton）物种组成、丰度与生物量数据。样本采集于多个离散水层：0-10m、0-20m、0-50m、10-25m、25-50m、50-100m，以及依据水体层化结构和温跃层深度确定的从底层至表层的水层。 所采集的样品采用Dimov（1959）方法开展分析。根据浮游动物密度将样品定容至25~30mL，并强力搅拌直至所有生物在样品体积内均匀随机分布。随后取5mL样品注入矩形计数框，用于分类鉴定与计数。体型大于1mm且丰度较低的物种以全样进行统计。在体视显微镜下使用Dimov计数框对生物进行计数与形态测量，直至达到最低可行分类单元。物种分类鉴定由阿森·康苏洛夫（Asen Konsulov）在海洋学研究所完成，参考了相关分类学专著（Mordukhay-Boltovskoy, F.D.（主编），1968、1969、1972）。生物量采用Petipa（1959）提出的方法以湿重（wet weight）估算，估算依据为物种特异性湿重。随后依据Vinogradov与Shushkina（1987）提出的换算公式DW=0.16×WW，将湿重转换为干重（dry weight）。 本次采集的样品同样采用Dimov（1959）方法处理。根据浮游动物密度将样品定容至25~30mL，充分搅拌至生物均匀随机分布后，取5mL样品注入矩形计数框，用于分类鉴定与计数。其中桡足类（Copepods）与枝角类（Cladoceras）需鉴定至具体物种并统计个体数量；其余中型浮游动物（mesozooplankters）仅需鉴定并统计至较高分类阶元（通常归类为中型浮游动物类群）。体型大于1mm且丰度较低的物种以全样进行统计。在体视显微镜下使用Dimov计数框对生物进行计数与形态测量，直至达到最低可行分类单元。物种分类鉴定由阿森·康苏洛夫在海洋学研究所完成，参考前述分类学专著（Mordukhay-Boltovskoy, F.D.（主编），1968、1969、1972）。生物量采用Petipa（1959）提出的方法以湿重估算，基于各物种的标准平均体重（单位：mg/m³）。湿重通过公式DW=0.16×WW转换为干重（Vinogradov ME, Sushkina EA, 1987）。