Effect of flg22 peptide on Arabidopsis seedling roots with endodermis-expressed FLS2 in fls2 mutant background

To compare transcriptional outputs of FLS2 and SGN3 pathways solely within the endodermis, we conducted comparative RNAseq analysis of pCASP1::FLS2 fls2 and wild-type seedling roots treated by flg22 and CIF2 respectively, at three timepoints (30, 120, 480 min) (CIF2 responses data previously published by Fujita et al 2020). The receptor mutant controls sgn3 and fls2, displayed minimal gene responses to respective peptides. Overall design: Comparing flg22 treatment to H2O treatment at 3 time points (30, 120, 480 min) on pCASP1::FLS2-GFP fls2 (endodermis-expressed FLS2) to assess endodermis-specific FLS2 immune responses in 5-day-old seedling roots. The fls2 receptor mutant is used as a control.