Genome-wide expression profiling analysis of a SMC6 mutant mouse model

The function of the SMC5-6 complex is less clear, but it has an important role in a variety of different DNA repair processes and in resolving recombination structures. Interestingly mutation at a highly conserved residue (Ser994) in the ATP hydrolysis motif in the SMC6 C-terminal domain, resulted in mice with a mild phenotype. Total RNA obtained from homozygote 4 male mutant mice was compared to 2-4 wild type or 2 heterozygote controls.SMC5-6复合体（SMC5-6 complex）的功能尚不完全明确，但它在多种DNA修复过程及重组结构解离过程中发挥着重要作用。值得注意的是，在SMC6 C端结构域（SMC6 C-terminal domain）的ATP水解基序（ATP hydrolysis motif）中一处高度保守的残基（丝氨酸994，Ser994）发生突变时，会使小鼠出现轻度表型。研究人员将从4只纯合雄性突变小鼠中提取的总RNA，与2-4只野生型或2只杂合型对照小鼠的总RNA进行了比对分析。