Various Enzymatic Treatments and Media yield Fluctuant Cell Cycle Resolutions of Yeast

The purpose of this study is to more deeply reveal the variation of cell cycle performance when yeast cells growing in different media.
Conclusion:
The cytometric resolutions were found significantly altered not only when the enzymatic services were extended but also when the growing medium was alternated from one to another, which resulted in various cell cycle performances.
Notes:
Saccharomyces cerevisiae, a species of yeast, is an important model organism and a typical fungal representative for studies on eukaryotes. Flow cytometry is an essential and efficient approach for the analysis of the cell cycle in yeast, and refining the cell cycle resolution is important. However, the resolution refinement is possibly subject to multiple factors, including altered enzymatic treatment and medium variation, which had been seldomly investigated. In this study, flow cytometry combined with multiple enzymatic treatments and various media are performed to determine how this resolution is affected. We show that the resolution is significantly altered under multiple enzymatic conditions. In addition, various media also results in the fluctuation in the resolution. Therefore, different enzymes are confirmed to refine the resolution with varied efficiencies, which is suspicious of due to the alternated culture media with different amino nitrogen concentrations, impacting the protein content in the yeast cell wall, and leading to the changed permeability of cell wall and the variable resolution. This study provides beneficial technical information about the influences of the adjustments of media and enzymes on refining the cell cycle resolution of yeast and, probably, on other fungi, which should be included in the consideration in future research. No gating was setup. The count axes of the histograms were all fixed to 2.0K. The algorithm to the cell cycle statistics was set to “Dean-Jett-Fox” for all the samples. Other settings were kept default.