Expression profiling of PBMCs from VA-ECLS patients. Expression profiling of PBMCs from VA-ECLS patients

We profiled RNA expression by means of single cell RNA-Seq from a total of ~60,000 cells from 38 patients undergoing VA-ECMO due to acute heart failure (including acute exacerbation of chronic failure). Samples were taken at the time of initiation of ECMO (mean +/- 79 minutes from cannulation). Overall design: Survival analysis, 30 day survival, with cross sectional analysis of gene expression at start of VA-ECLS Submitters plan to submit raw files to dbGaP to protect patient sequence privacy