Antimicrobial Potential of Terpene Isolates of Cannabis sativa against Staphylococcus pseudintermedius: A Novel Approach to Combat Antimicrobial Resistance in Veterinary Medicine: raw data

Staphylococcus pseudintermedius is a harmful bacterium that affects dogs, causing various infections like canine pyoderma, otitis externa, and systemic issues in the urinary, respiratory, and reproductive systems. Canine pyoderma, an infection of a dog's skin and hair follicles, is a leading reason for antibiotic prescriptions in dogs. The excessive prescription of antibiotics has resulted in a rise in multidrug-resistant S. pseudintermedius and the emergence of methicillin-resistant S. pseudintermedius (MRSP) which pose significant challenges in treating infections, highlighting the need for alternative therapeutic strategies. Given the rising antimicrobial resistance (AMR) and limited antibiotic options, the potential of plant-derived terpenes as novel antimicrobial agents is being explored. Here we investigated the antimicrobial efficacy of eight terpenes commonly found in Cannabis sativa against clinical isolates of S. pseudintermedius. The study evaluates the antibacterial and bactericidal activities of these terpenes, their effects on bacterial ultrastructure, and time-kill kinetics. Among the terpenes tested, nerolidol emerged as particularly effective, demonstrating potent antibacterial activity and low cytotoxicity, with its efficacy influenced by its hydrophobicity and hydroxyl group positioning. Our findings contribute to understanding the antibacterial action of Cannabis terpenes, underscoring their potential as innovative solutions in veterinary medicine for combating drug-resistant pathogens such as S. pseudintermedius.

中间葡萄球菌 (Staphylococcus pseudintermedius) 是一种可感染犬只的有害病原菌，可引发多种感染病症，包括犬脓皮症、外耳炎，以及泌尿、呼吸与生殖系统的全身性感染。犬脓皮症作为犬皮肤及毛囊的感染性疾病，是犬类抗生素处方开具的主要诱因之一。抗生素的过度使用导致多重耐药性中间葡萄球菌的数量攀升，并催生了耐甲氧西林中间葡萄球菌 (MRSP, methicillin-resistant S. pseudintermedius)，这类耐药菌给感染治疗带来了极大挑战，凸显了开发替代性治疗策略的迫切需求。鉴于抗菌药物耐药性 (antimicrobial resistance, AMR) 问题日益严峻，且可用抗生素种类有限，植物来源的萜类化合物作为新型抗菌剂的研发潜力正受到广泛关注。本研究针对大麻 (Cannabis sativa) 中常见的8种萜类化合物，开展了其对临床分离株中间葡萄球菌的抗菌活性评估。本研究考察了这些萜类化合物的抗菌与杀菌活性、对细菌超微结构的影响，以及时间-杀菌动力学特征。在所测试的萜类化合物中，橙花叔醇 (nerolidol) 表现尤为突出，其具备强效抗菌活性且细胞毒性较低，其抗菌效能受自身疏水性与羟基取代位置的影响。本研究结果有助于解析大麻来源萜类化合物的抗菌作用机制，凸显了其作为兽医临床创新治疗手段的潜力，可用于对抗诸如中间葡萄球菌这类耐药病原菌。