Mutation frequencies induced by microinjection using various concentrations of Cas9 mRNA/gRNA mix.
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*Mutant lines subjected to sequencing of Dma-ey loci. In Figure 2B, two mutants named m1 and m2 were from 4 mutants injected with 50 ng/µL each of gRNA and 500 ng/µL Cas9 mRNA (50 each, 500), m3 and m4 from 2 mutants (150 each, 1,000), and m5 from 1 mutant (150 each, 2,000).
创建时间：
2014-05-30
