Twist - torsion coupling in beating axonemes

The dataset published here was used to measure a high resolution 3D wavefom of isolated and reactivated axonemes from Chlamydomonas reinhardtii.Note: This dataset contains a motion-blur correction appled to to the data in doi: https://doi.org/10.1101/2024.03.18.585533 and code that details how the 3D average waveform was calulated.

It was further used to show twist-torsion coupling in these axonemes (doi:10.1038/s41567-025-02783-2).

The data is organized in seven folders:

1) high resolution average 3D waveform of isolated and reactivated axonemes from Chlamydomonas Reinhardtii.Data files (MATLAB and txt format) contain the 3D coordinates (along the 3D arc-length) of 32 axonemal shapes that comprise one beat-cycle. A corresponding txt file describes the details of the dataset. 

2) 3D waveforms of single isolated and reactivated axonemes from Chlamydomonas Reinhardtii.Data files (MATLAB and txt format) contain the 3D shapes of 17 individual axonemes obtained from defocused darkfield-microsopy images. A corresponding txt file describes the details of the dataset.

3) Image Raw Data of single isolated and reactivated axonemes used to reconstruct the 3D waveformMovie files (multi-layer tif) of reactivated axonemes imaged with defocused-darkfield-microscopy. A corresponding txt file describes the details of the dataset.

4) Calibration of defocused darkfield-microscopy. Data file (MATLAB) contains the relationship between the z-position relative to the focal plane and the full-width-at-half-maximum (FWHM) of the axoneme signal, measured normal to the centerline as well as the z-stack of imges (multi-layer tif) used to extract this relation. A corresponding txt file describes the details of the dataset.

5) Distance between gold nano paricle (GNP) and the axonemal centerline as a function of the beat cycleData file (MATLAB) contains 20 measurements of d_C (where d_C is the normal distance between the center position of the GNP and the axoneme centerline in 2D images) as a function of time. A corresponding txt file describes the details of the dataset.

6) Image Raw Data of single isolated and reactivated axonemes with attached GNPs used to measure d_C. Movie files (multi-layer tif) of reactivated axonemes with attached gold nano particles (GNPs) imaged with darkfield-microscopy. A corresponding txt file describes the details of the dataset.

7) Code to calculate the average 3D waveform from defocused darkfield moviesMATLAB code used to calculate the average waveform of an axoneme that was recorded with high-speed defocused darkfield microscopy.A corresponding pdf file (Manual.pdf) describes the details of the procedure in 6 steps.