Material from PhD thesis: Appendix A-3 RPA primers and crRNA
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资源简介：
This dataset contains supplemental material as part of a PhD thesis: Development of Novel Diagnostic Tools and Antigen Discovery for Devil Facial Tumour Disease.
The material contains a list of recombinase polymerase amplification (RPA) primers and LwCas13a CRISPR RNA (crRNA) for the amplification and detection of DFT1, DFT2, RPL13a, and ZIKV. The material also includes a list of primer combinations. The best-performing primer pair and crRNA for each target are indicated. These primers and crRNA were used in the development of CRISPR-based point-of-care diagnostic tools, termed specific high-sensitivity enzymatic reporter unlocking (SHERLOCK), to identify DFT1 and DFT2 in the field with limited instrumentation.
提供机构：
University of Tasmania, Australia
