Material from PhD thesis: Appendix A-3 RPA primers and crRNA

This dataset contains supplemental material as part of a PhD thesis: Development of Novel Diagnostic Tools and Antigen Discovery for Devil Facial Tumour Disease. The material contains a list of recombinase polymerase amplification (RPA) primers and LwCas13a CRISPR RNA (crRNA) for the amplification and detection of DFT1, DFT2, RPL13a, and ZIKV. The material also includes a list of primer combinations. The best-performing primer pair and crRNA for each target are indicated. These primers and crRNA were used in the development of CRISPR-based point-of-care diagnostic tools, termed specific high-sensitivity enzymatic reporter unlocking (SHERLOCK), to identify DFT1 and DFT2 in the field with limited instrumentation.本数据集为一篇博士学位论文的补充材料，该论文主题为《塔斯马尼亚恶魔面部肿瘤病（Devil Facial Tumour Disease, DFTD）的新型诊断工具开发与抗原发现》。本材料包含用于扩增和检测DFT1、DFT2、RPL13a及寨卡病毒（ZIKV）的重组酶聚合酶扩增（recombinase polymerase amplification, RPA）引物与LwCas13a CRISPR RNA（crRNA）清单，同时收录了引物组合列表。文中标注了针对各检测靶标表现最优的引物对与crRNA。上述引物与crRNA被用于开发基于CRISPR的即时诊断工具——特异性高灵敏度酶报告解锁（specific high-sensitivity enzymatic reporter unlocking, SHERLOCK），以在仪器设备有限的野外环境中实现DFT1与DFT2的田间鉴定。