Data from: β-aminobutyric acid-induced resistance in postharvest peach fruit involves interaction between the MAPK cascade and SNARE13 protein in salicylic acid-dependent pathway

The inducer β-aminobutyric
acid (BABA) is capable of immune
response in various plants. However, the specific
mitogen-activated protein kinase (MAPK) cascade involved in
BABA-induced resistance (BABA-IR) has not yet been elucidated.
Here, peach fruits treated with the BABA exhibited a
pattern-triggered immunity (PTI) defense
against Rhizopus stolonifer, accompanied by the generation of
reactive oxygen species (ROS) and activation of MAPK
cascade. Transcriptome sequencing suggested a total of
fifteen PpMAPKKK/PpMAPKK/PpMAPK genes involved in
BABA-IR in peach fruit.
Further qRT-PCR analysis showed that the transcript
profiles of PpMAPKKK3,
PpMAPKK5 and PpMAPK1 were obviously
potentiated. Subsequently, yeast two-hybrid (Y2H), luciferase
complementation imaging (LCI), pull-down and in vitro
phosphorylation assays were conducted to characterize the
complete MAPK cascade (PpMAPKKK3-PpMAPKK5-PpMAPK1) involved in peach
fruit. Moreover, the downstream events of MAPK1 include the involvement
of SNARE13 and the
corresponding NPR1-responsive defense.
Single silencing of MAPKKK3, MAPKK5 or MAPK1 and double silencing
of MAPKKK3/MAPKK5 or MAPKK5/MAPK1 resulted in enhanced susceptibility to
the fungus R. stolonifer in mutants and attenuated salicylic
acid (SA)-dependent defense gene expression; in contrast, the
homologous or heterologous overexpression of
PpSNARE13 in peach fruit or Arabidopsis led to an
enhanced SA pool and elevated expression of PR genes.
Reciprocally, the ppsnare13cas9 mutants are generally
compromised in the priming of
SA-dependent resistance. Therefore, the
MAPKKK3-MAPKK5-MAPK1 cascade contributes to PTI signal
transduction in BABA-elicited peach fruit, by combination with
downstream events such as SNARE13, NPR1, and SA-dependent signaling.