Caudoviricetes strain:vB_SmaS-Bfi_2 Genome sequencing and assembly

S. maltophilia phage Bfi2 (vB_SmaS-Bfi_2) was isolated from soil. Negative staining TEM of Bfi2 indicated that is a siphovirus within the class Caudoviricetes, possessing an icosahedral capsid and a long, flexible, non-contractile tail. Bfi2 demonstrated a moderate host range, with the ability to infect (6/11) 55% of S. maltophilia strains, and was stable in temperature and pH conditions similar to those found in vivo. Moreover, Bfi2 significantly inhibited biofilm formation of its host strain S. maltophilia K279a. Bfi2 was shown have a relatively high adsorption efficiency, and a latent period of ~ 60 minutes with a burst size of ~ 33 PFU/cell. Bfi2 may be a suitable candidate for an alternative to the use of antibiotics and our study has contributed to our knowledge of the diversity of S. maltophilia phages.