Single cell transcriptomics reveal an essential role for Hippo signaling in cell state transitions during cardiac fibroblast development

We use single cell RNA-sequencing (Drop-seq) to profile cardiac cells (excluded atria) from control (Wt1 CreERT2/+; Rosa26mTmG/+) and epicardial-deficient Lats1/2 kinase embryonic hearts (Wt1 CreERT2/+; Lats1/2 F/F; Rosa26mTmG/+) at E13.5 and E14.5. Each experimental group includes cells sampled from four embryonic hearts. Drop-seq of e13.4 and e14.5 Control and Wt1-CreER; Lats1/2 fl/fl cardiac tissue.