Single-cell RNA sequencing profiling of periarticular tissue from epidermal-specific SPRY1-deficient (Spry1 cKO) mice and control mice

Spry1 cKO mice were found spontaneously developed psoriasis-like skin inflammation and arthritis.To further investigate the periarticular immune microenvironment in paws, single-cell RNA sequencing of the periarticular tissue from Spry1 cKO and control mice was performed. Overall design: The periarticular tissue of Spry1 cKO mice and control mice were harvested after tamoxifen administration, and single-cell suspensions of periarticular tissue were prepared using collagenase IV. To obtain sufficient immune cells for analysis, four mice were pooled as one sample for the control group (12 control mice in total for n=3), and then CD45+ and CD45- cells were sorted and mixed at a ratio of 3:1 in both groups for scRNA-seq analysis.