DNA adducts form in mouse lung and liver after oral naphthalene exposure

Naphthalene is a ubiquitous environmental contaminant and suspected carcinogen that forms DNA adducts in tissue explants, which is a mechanism of concern for genotoxicity, but it was not known if naphthalene could form DNA adducts in vivo. Naphthalene-DNA adducts are small and difficult to detect, so we utilized accelerator mass spectrometry to detect adducts with high sensitivity.  Wild-type C57BL/6 mice were orally exposed to 50 mg/kg 14C-naphthalene, lung and liver were collected 2, 4, 24, or 72 hours post-exposure, then DNA was isolated from the tissue. DNA was then processed for accelerator mass spectrometry analysis following standard protocols, explained in detail in Domanico et al. (2025)(doi.org/10.1093/toxsci/kfaf017). Resulting measurements of 14C signal in these DNA samples comprise this dataset, and can be used to calculate the level of DNA adduct formation in tissue. We detected naphthalene-DNA adducts at various timepoints post-exposure, with levels decreasing over t..., Accelerator mass spectrometry (AMS) was used to detect stable DNA adducts formed with murine lung and liver DNA following an in vivo oral exposure to radiolabeled 14C-naphthalene. Dosing solutions of 14C-naphthalene were prepared by diluting stock 14C-naphthalene (Moravek Inc.) with unlabeled naphthalene (Sigma-Aldrich) to reach a lower specific activity acceptable for use with AMS. Wild-type C57BL/6 mice were exposed to the 14C-naphthalene dosing solution or a corn oil vehicle via oral gavage; mouse lung lobes and liver were then collected 2, 4, 24, or 72 hours post-exposure. Tissue was homogenized and DNA was extracted via silica adsorption (DNeasy Kit; Qiagen), with particular care taken to remove proteins, as naphthalene also forms protein-DNA adducts. DNA purity was confirmed by Nanodrop before proceeding with AMS processing. DNA samples were then dried in a quartz vial, combined with tributyrin carbon carrier, and combusted to yield filamentous carbon. AMS was used to measure Carb..., # DNA adducts form in mouse lung and liver after oral naphthalene exposure

Dataset DOI: [10.5061/dryad.51c59zwmf](10.5061/dryad.51c59zwmf)

## Description of the data and file structure

Accelerator mass spectrometry (AMS) was used to detect stable DNA adducts formed with murine lung and liver DNA following an in vivo oral exposure to radiolabeled 14C-naphthalene. Dosing solutions of 14C-naphthalene were prepared by diluting stock 14C-naphthalene (Moravek Inc.) with unlabeled naphthalene (Sigma-Aldrich) to reach a lower specific activity acceptable for use with AMS. Wild-type C57BL/6 mice were exposed to the 14C-naphthalene dosing solution or a corn oil vehicle via oral gavage; mouse lung lobes and liver were then collected 2, 4, 24, or 72 hours post-exposure. Tissue was homogenized and DNA was extracted via silica adsorption (DNeasy Kit; Qiagen), with particular care taken to remove proteins, as naphthalene also forms protein-DNA adducts. DNA purity was confirmed by Nanodrop before pr...,