DNA adducts form in mouse lung and liver after oral naphthalene exposure

Naphthalene is a ubiquitous environmental contaminant and suspected carcinogen that forms DNA adducts in tissue explants, which is a mechanism of concern for genotoxicity, but it was not known if naphthalene could form DNA adducts in vivo. Naphthalene-DNA adducts are small and difficult to detect, so we utilized accelerator mass spectrometry to detect adducts with high sensitivity.  Wild-type C57BL/6 mice were orally exposed to 50 mg/kg 14C-naphthalene, lung and liver were collected 2, 4, 24, or 72 hours post-exposure, then DNA was isolated from the tissue. DNA was then processed for accelerator mass spectrometry analysis following standard protocols, explained in detail in Domanico et al. (2025)(doi.org/10.1093/toxsci/kfaf017). Resulting measurements of 14C signal in these DNA samples comprise this dataset, and can be used to calculate the level of DNA adduct formation in tissue. We detected naphthalene-DNA adducts at various timepoints post-exposure, with levels decreasing over t..., Accelerator mass spectrometry (AMS) was used to detect stable DNA adducts formed with murine lung and liver DNA following an in vivo oral exposure to radiolabeled 14C-naphthalene. Dosing solutions of 14C-naphthalene were prepared by diluting stock 14C-naphthalene (Moravek Inc.) with unlabeled naphthalene (Sigma-Aldrich) to reach a lower specific activity acceptable for use with AMS. Wild-type C57BL/6 mice were exposed to the 14C-naphthalene dosing solution or a corn oil vehicle via oral gavage; mouse lung lobes and liver were then collected 2, 4, 24, or 72 hours post-exposure. Tissue was homogenized and DNA was extracted via silica adsorption (DNeasy Kit; Qiagen), with particular care taken to remove proteins, as naphthalene also forms protein-DNA adducts. DNA purity was confirmed by Nanodrop before proceeding with AMS processing. DNA samples were then dried in a quartz vial, combined with tributyrin carbon carrier, and combusted to yield filamentous carbon. AMS was used to measure Carb..., # DNA adducts form in mouse lung and liver after oral naphthalene exposure Dataset DOI: [10.5061/dryad.51c59zwmf](10.5061/dryad.51c59zwmf) ## Description of the data and file structure Accelerator mass spectrometry (AMS) was used to detect stable DNA adducts formed with murine lung and liver DNA following an in vivo oral exposure to radiolabeled 14C-naphthalene. Dosing solutions of 14C-naphthalene were prepared by diluting stock 14C-naphthalene (Moravek Inc.) with unlabeled naphthalene (Sigma-Aldrich) to reach a lower specific activity acceptable for use with AMS. Wild-type C57BL/6 mice were exposed to the 14C-naphthalene dosing solution or a corn oil vehicle via oral gavage; mouse lung lobes and liver were then collected 2, 4, 24, or 72 hours post-exposure. Tissue was homogenized and DNA was extracted via silica adsorption (DNeasy Kit; Qiagen), with particular care taken to remove proteins, as naphthalene also forms protein-DNA adducts. DNA purity was confirmed by Nanodrop before pr...,# 口服萘暴露后小鼠肺与肝脏中形成DNA加合物 数据集DOI：[10.5061/dryad.51c59zwmf](10.5061/dryad.51c59zwmf) ## 数据集概述 萘是一种广泛分布的环境污染物，疑似致癌物，可在离体组织中形成DNA加合物，这是其引发遗传毒性的潜在机制，但此前尚未明确萘在体内是否能够形成DNA加合物。萘-DNA加合物体积微小且难以检测，因此本研究采用加速器质谱（accelerator mass spectrometry，AMS）以实现高灵敏度的加合物检测。 野生型C57BL/6小鼠经口暴露于50mg/kg的¹⁴C-萘，分别于暴露后2、4、24或72小时采集肺与肝脏组织，随后从组织中分离DNA。按照Domanico等人（2025年，doi.org/10.1093/toxsci/kfaf017）中详细说明的标准流程，对DNA进行加速器质谱分析。本数据集包含上述DNA样品中¹⁴C信号的检测结果，可用于计算组织中DNA加合物的形成水平。本研究在暴露后的多个时间点均检测到萘-DNA加合物，其水平随时间呈下降趋势…… 本研究采用加速器质谱（AMS）检测经体内口服暴露于放射性标记¹⁴C-萘后，小鼠肺与肝脏DNA中形成的稳定DNA加合物。¹⁴C-萘给药溶液的配制方式为：将储备液¹⁴C-萘（Moravek Inc.）与未标记萘（Sigma-Aldrich）混合稀释，以获得适配加速器质谱检测的较低比活度。野生型C57BL/6小鼠经口灌胃给予¹⁴C-萘给药溶液或玉米油溶剂对照，分别于暴露后2、4、24或72小时采集小鼠肺叶与肝脏组织。将组织匀浆后，通过硅胶吸附法（DNeasy Kit；Qiagen）提取DNA，由于萘同样可形成蛋白质-DNA加合物，因此实验中特别注意去除蛋白质杂质。在进行加速器质谱处理前，通过Nanodrop检测确认DNA纯度。将DNA样品置于石英瓶中干燥，加入三丁酸甘油酯碳载体后进行燃烧，以获得丝状碳。随后采用加速器质谱测量碳的…… ## 数据与文件结构说明 本研究采用加速器质谱（AMS）检测经体内口服暴露于放射性标记¹⁴C-萘后，小鼠肺与肝脏DNA中形成的稳定DNA加合物。¹⁴C-萘给药溶液的配制方式为：将储备液¹⁴C-萘（Moravek Inc.）与未标记萘（Sigma-Aldrich）稀释混合，以获得适配加速器质谱检测的较低比活度。野生型C57BL/6小鼠经口灌胃给予¹⁴C-萘给药溶液或玉米油空白溶剂，分别于暴露后2、4、24或72小时采集肺叶与肝脏组织。将组织匀浆后，通过硅胶吸附法（DNeasy Kit；Qiagen）提取DNA，鉴于萘亦可形成蛋白质-DNA加合物，实验过程中特别注重去除蛋白质杂质。在开展加速器质谱分析前，通过Nanodrop仪确认DNA纯度。将DNA样品置于石英瓶中干燥，添加三丁酸甘油酯碳载体后进行燃烧，以制备丝状碳样品，随后采用加速器质谱对碳含量……