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Supporting figures.

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https://figshare.com/articles/dataset/Mutations_in_Planar_Cell_Polarity_Gene_SCRIB_Are_Associated_with_Spina_Bifida/756216
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Figure S1. Transfection efficiency calculation: a. count total cells number (n) by Operetta; b. selected and counted GFP positive cells number (a). Transfection efficiency = (a/n) *100%. Figure S2. GFP-SCRIB constructs transfection efficiency. No significant difference was detected between SCRIB wild type and the mutant. Figure S3. GFP-SCRIB constructs transfected GFP-positive cells fluorescence intensity. a. individual GFP-positive cell (green dot) green fluorescence intensity, red dot indicate GFP-negative cells. b. Different GFP-SCRIB constructs GFP-positive cells average fluorescence intensity. (DOCX)
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