NCBI Sequence Read Archive (SRA) accession numbers for fastq sequence files of 16S amplicons from a longitudinal study of SCTLD at St. John, U.S. Virgin Islands from 2020 to 2024

In St. John, U.S. Virgin Islands, a longitudinal study of Colpophyllia natans was conducted. Six corals per site (two sites) were tagged and repeatedly sampled from July 2020 through March 2024, during which time, stony coral tissue loss disease (SCTLD) arrived on the reefs. As the tagged corals contracted SCTLD, coral tissue and near-coral seawater (2-5 centimeters from colony surface) were collected. Coral tissue was separated from the coral skeleton, and the seawater was filtered through 0.2-micrometer (µm) filters. DNA was extracted from the tissue and filters, and 16S ribosomal RNA gene sequencing was done to determine the bacteria and archaea communities that live within and near the corals. The goal of the study was to determine how coral-associated microbiomes are affected by the arrival of SCTLD through time. This data set contains the fastq files of the 16S amplicon sequencing of the coral tissue and near-coral seawater communities. National Center for Biotechnology Information (NCBI) SRA: SRP550314; BioProject: PRJNA1194595本研究在美国美属维尔京群岛圣约翰岛开展了针对叶状珊瑚（Colpophyllia natans）的纵向研究。在两个采样点位中，每个点位标记6株珊瑚，并于2020年7月至2024年3月期间进行重复采样；在此期间，石珊瑚组织损失病（stony coral tissue loss disease, SCTLD）传入该礁区。当标记珊瑚感染SCTLD后，研究人员采集了珊瑚组织以及距珊瑚群落表面2-5厘米的近珊瑚海水样本。随后将珊瑚组织与珊瑚骨骼分离，并将采集的海水通过0.2微米（µm）滤膜进行过滤。从珊瑚组织与滤膜中提取DNA，并通过16S核糖体RNA基因测序，解析珊瑚体内及周边的细菌与古菌群落组成。本研究的核心目标是阐明SCTLD传入随时间推移对珊瑚相关微生物组的影响。本数据集包含珊瑚组织与近珊瑚海水群落的16S扩增子测序fastq文件。美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）SRA编号：SRP550314；BioProject编号：PRJNA1194595