Regional specialization and fate specification of mesenchymal stromal cells in skeletal development [scRNA-seq]

To identify cellular heterogeneity and molecular signatures of bone marrow stromal (BMSCs) cells, we performed scRNA-seq of non-hematopoietic stromal cells from 3-week-old mice. In addition, to gain further insight into MSC differentiation in vivo, Pdgfrb-CreERT2 Rosa26-mTmG (Pdgfrb-Cre R26-mT/mG) double transgenic mice were treated with tamoxifen at P1-3 and sacrificed at P21 for the isolation of GFP+ cells from long bone followed by scRNA-seq analysis. Single cell -RNA sequencing of non-hematopoietic bone stromal cells (BSC) postnatal day 21 old bone and PdgfrbCre-R26 mTmG GFP+ sorted cells using 10X genomics.