circRNA sequence authenticity in live cells

Exogenous RNA, such as circRNA, could be edited by endogenous editors, such as C-to-U editor activation-induced deaminase and apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (AID/APOBEC), and A-to-I editor Adenosine deaminases acting on RNA (ADAR) (I: inosine, recognized as G). Editing of circRNA may interfere with IRES and Kozak sequence to initiate antigen translation, and may alter antigen products or mutate stop codons. Using circRNA sequencing (circRNA-seq) followed by bioinformatic circRNA mapping, we monitored the sequence integrity of circRNA-SIINFEKL after transfection in live mouse bone marrow dendritic cells (BMDCs) for 24 h.