Time-course transcriptomic profiling of cell-cycle entry in MCF10A cells

To identify potential novel regulators of G1/S transition and S-phase progression, we performed an RNA sequencing (RNA-Seq) time-course analysis of non-transformed human breast epithelial cells (MCF10A) released into the cell cycle from serum starvation, in the presence of DMSO or a specific CDK4/6 inhibitor palbociclib. Overall design: Conditions include: 0hr, 3hr DMSO, 6hr DMSO, 9hr DMSO, 9hr palbociclib, 12hr DMSO, 12hr palbociclib, 15hr DMSO, 15hr palbociclib, 24hr DMSO, 24hr palbociclib. A total of 31 samples containing triplicates (except 24-hour time point, which was performed in duplicates) were analyzed.