High-throughput propagation of human prostate tissue from induced-pluripotent stem cells.

We demonstrate a new high-throughput model where rapidly proliferating and easily handled induced pluripotent stem cells enable generation of human prostate tissue in vivo and, for the first time, in vitro. Using a co-culture technique with urogenital sinus mesenchyme, we recapitulated the in situ prostate histology, including the stromal compartment and the full spectrum of epithelial differentiation. Overall design: Examination of transcripts in IPSC, Definitive Endoderm and Prostate Pro Organoids at 12 Weeks through RNA-seq analysis