Expression data from mouse sarcoma tumor cell lines

Vanin1, a regulator of vitamin B5 metabolism, is expressed by sarcoma tumors. We evaluated its impact on sarcoma growth by using sarcoma cell lines derived from p16p19Vnn1-deficient mice and further transduced with an oncogenic RasV12 oncogene (R tumors) in the presence or not of a catalytically active (VR tumors) or mutated (VdR tumors) Vnn1 isoform. We used microarrays to detail the global programme of gene expression associated with the growth potential of various tumor cell lines grafted in Nude mice Tumor cell lines were injected in Nude mice under normal diet (nd) and harvested between 2 and 3 weeks of growth. After RNA extraction, analysis of the transcriptome was perfomed using an Affymetrix platform at the Strasbourg